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A new method has been developed for the simultaneous measurement of prostaglandins (PGs) and ecdysteroids in insects by high-performance liquid chromatography (HPLC) using fluorescence labeling. PG and ecdysteroid containing fractions were obtained from insects by passing a crude extract through a Sep-pak C18 cartridge then through piperidinohydroxypropyl Sephadex LH-20. The PGs and ecdysteroids were then treated with 1-anthroyl nitrile and measured by HPLC analysis on a C18 column with a fluorescence detector, the limit of detection being 10 pg of injected standard derivatives. This method can be used for PGs and ecdysteroids from an individual organ or from the haemolymph of an individual insect with satisfactory sensitivity.

Simultaneous measurement of prostaglandins and ecdysteroids in insects by high-performance liquid chromatography with fluorescence labeling.