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iheasurement of NAD (NAD' + NADH) b}, Nisselbaum's method2} has been successfully used for the estimation of blood NAD and that blood NAD values varied depending on the niucin contents i] the diets of rats,i) That is, the measurement of blood NAD can be used as a biologic index for niacin nutrition. In this papcr, we report on a simple method for measuring blood NADP (NADP' + NADPH), and blood ]evels of NAD and NADP in hcalthy women. Phenazine methosulfate (PMS), nicotinamide, and glycylglycine were obtained from Wako Purc Chemical Industrics. 3-(4,5-Dimcthyl-2-thiazolyl)-2,5-diphenyl-2Htetrazolium bromide (MTT) was obtained from Dojin Chernical Laboratories Co.. Ltd. Yeast alcohol dehydrogenase, yeast glucose-6-phosphate dehydrogenase, NAD', NADH, NADPT, NADPH, and glucose-6phosphate were from Oriental Ycast Co., Ltd, Other chcmicals were used ar the highest purity obtainable from

Simple measurement of blood NADP and blood levels of NAD and NADP in humans.