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When sweet potato root tissue is sliced and incubated, much synthesis of chiorogenic acid (3-caffeoylquinic acid) and isochlorogenic acid (isomers of dicaffeoylquinic acid) takes place. l ~4) Our earlier tracer experiment 4 ) suggested that chlorogenic acid was directly converted to isochloro­ genic acid through a one-step reaction in injured sweet potato root. This communication deals with the detection of the enzyme activity in sweet potato root which catalyzes the conversion of chlorogenic acid to isochlorogenic acid (3,5­ dicaffeoylquinic acid). Acetone powder was prepared from sweet potato (Ipomoea hatatas Lam, cv, Norin 1) root slices, 0.5 cm thick, which had been incubated at 25°C for 48 hr. The powder (3 g) was extracted with 30 ml of 0.1 M phosphate

agricultural and biological chemistry

An enzyme in sweet potato root which catalyzes the conversion of chlorogenic acid, 3-caffeoylquinic acid, to isochlorogenic acid, 3,5-dicaffeoylquinic acid.