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Maltopentaose-producing microorganisms were screened from a wide variety of sources. A bacterium which produced maltopentaose as the main product from a culture medium containing soluble starch was isolated from soil and identified as Bacillus cereus from its morphological, physiological, and biochemical properties. The culture conditions for the production of maltopentaose were investigated. The optimum medium consisted of 2.5 to 8% soluble starch, 1.5% peptone, and 0.25% NaCl. Under the optimum conditions of cultivation, the ratio of maltopentaose produced was in the range of 29 to 47% to the residual sugar.

agricultural and biological chemistry

Studies on production of maltopentaose by Bacillus cereus NY-14. Part I. Characterization of a maltopentaose-producing bacterium and its cultural conditions.