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this extract, and identified by spectroscopic methods. We now describe the isolation, activity and structure elucidation of these alkaloids. An analysis of proton-proton connectivities by high resolution GOSY experiments is also reported. The crude methanol extract was dispersed into water and the aqueous suspension was extracted with hexane, ether and ethyl acetate. Each of these fractions was monitored by the previously described assay for molluscicidal activity.5) The ether extract showedactivity and was further fractionated by silica gel column chromatography (0.5% CH3OH-CHCI3) followed by reversed-phase (C18) liquid chromarography (70% CH3OH-H2O) to give two major components (1, 2), one of which (2) showed strong molluscicidal activity. The non-active component (1) was obtained as colorless crystals, mp 265°C (EtOH), [a]^0

Quinolizine alkaloids from the African medicinal plant Calpurnia aurea: Molluscicidal activity and structural study by 2D-NMR.