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Since the discovery of brassinolide (1) from rape pollen1} a variety of novel brassinoliderelated plant growth regulators have been found from chestnut insect gall (compounds 52) and 83)), Dolichos lablab (2, 3, 6 and 7)4-6) phaseolus vulgaris (10 and ll),7) Brassica campestris (4, 8 and 9) and Thea sinensis (8 and 9).3) Our recent investigation led to the isolation of a new brassinoliderelated steroid (12) from pollen of Pinus Thubergii Parl. The Pinus pollen (19kg) was processed by the following procedure to afford crystals of 12 (1.7mg, prisms), mp 231~233°C: 1, iso-

2-Deoxycastasterone, a new brassinolide-related bioactive steroid from Pinus pollen.