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yields of gene products are usually achieved by using cloning vehicles capable of copy number change. The number of copies of such vehicles can be increased conveniently by means of thermal induction. Phage X vehicles with a temperature-sensitive c\ gene product are reported to be useful for convenient amplification.1>3) Plasmid vehicles derived from a socalled runaway-replication mutant have also been reported to replicate without copy number control above 35°C.4) In this communi-

Construction of "sleeper" cloning vehicles for enzyme overproduction.