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disodium phosphate, 0.005 m disodium EDTAdihydrate, pH 8.0). Following recentrifugation, 10g of wet cell paste was dissolved with vortex mixing in 10 ml ofPE buffer. To the cell suspension, 10ml of reagent ACSgrade DMSO (Eastman Organic Chemicals, Rochester, N. Y.) was added dropwise with vortex mixing. An exothermic reaction of solvation occurred, bringing the temperature of the mixture to about 30°C. After 10 min mixing, the cells were centrifuged at 4°C, the supernatant decanted, and the cell pellet twice resuspended in 10ml PE buffer and

Pretreatment of cells of Klebsiella pneumoniae with 50% (V/V) dimethylsulfoxide yields purified deoxyribonucleic acid of low polysaccharide content.