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torious pest whose larvae injure younger leaves near the growing point ofdaikon, cabbage and other cole crops. The chemical structure of the sex pheromone of this female has not previously been known. This paper reports the identification of its sex pheromone by means of the electroantenogram (EAG) technique1} and gas-liquid chromatography combined with mass spectrometry in a selected ion current monitoring mode (GC-SICM). The whole bodies of about 200 virgin females were immersed in rc-hexane for a few hours. This crude extract was fractionated by preparative TLC on a silica gel plate using benzene-ethyl acetate (15 : 1) as a developing solvent. It has already been shown that the functionality of the sex pheromone in Lepidoptera can be determined by its i?/-value on a silica gel TLC.2) Strong EAGactivity was observed in the acetate and aldehyde fraction (7?/0.5 ~0.7), but all other fractions, including the alcohol fraction (Rf 0.1-0.2), did not show this clear activity. The sex pheromonecomponentof this species was shownto be aldehyde and not acetate by the following experiments. The EAG-active material which was obtained retained its activity during alkaline hydrolysis, its activity being observed only at the acetate and aldehyde zone on TLC. Furthermore, acetylation of the alcohol fraction obtained from the hydrolysis products failed to regenerate the activity. On the other hand, the original activity was lost by treatment with sodium borohydride. The EAG activity was regenerated by oxidation of the alcohol fraction obtained from the reduction products with pyridinium chlorochromate.3)

Identification of the female sex pheromone of the cabbage webworm.