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Antigenicity of mannan preparation obtained by the usual method so far published and that prepared by our method was investigated with the rabbit antiserum against the intact yeast cell. The antigenicity of the former was a little less than the latter, but both mannan preparations were much less in the antigenicity as compared with the intact yeast cell. Thus the preparation of an intact surface antigen was attempted through treating the yeast whole cells successively with acetic acid, pepsin, acetone and ether. The cell envelope obtained by the procedure was almost intact microscopically and maintained the antigenic activity similar to that of the intact cells. The dry weight of the cell envelope was a half of the whole cell and its composition was sugar 60%, crude protein 32% and lipid 5%.

agricultural and biological chemistry

Immunochemical studies on surface of yeast cell. III. Antigenicities of several cell wall mannan preparations and cell envelope preparations from baker's yeast.