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presence of such saccharides. A cyclodextrin molecule is said to have a tubular form with an inner diameter of 6 A in a-cyclodextrin (ƒ¿-CD) or 7.5 A in ƒÀ-cyclodextrin (ƒÀ-CD). Both cyclodextrins are 7 A long and the inner side of the tube is rather basic and hydrophobic. Amylose is known to have a helical form with a helix consisting of six glucose units in the aqueous solution in the presence of n-butanol or thymol resembling ƒ¿ -CD rather than ƒÀ-CD . Amylose was also reported to have a helical form in dimethylsulfoxide solution .4) Many phenolic substances can easily form their inclusion complexes with cyclodextrin if their molecular sizes fit the hole.1,2) Since anthocyanins have phenolic moeities, we were interested in whether or not anthocyanin could form an inclusion complex with cyclodextrin. Three anthocyanins were examined: callistephin (pelargonidin-3-glucoside), chrysanthemin (cyanidin-3 glucosidc) and nasunin5) [delphindin-3-(4-(p-coumaroyl) L-rhamnosyl-(1,6)-glucosido)-5-glucoside]. Callistephin was extracted from the fresh fruits of strawberry (Fragaria chiroensis Duch. var. annassa Baillon). Chrysanthemin was extracted from the seeds of black soy bean (KUROMAME) (Glycine max Merill). Nasunin was ex tracted from the fruits of egg plant (Solanum melongena L .). These three anthocyanins extracted were proved to be pure by paper chromatography after some preparation procedure.6) ƒ¿-CD and ƒÀ-CD used were commercially available

Formation of an inclusion complex of anthocyanin with cyclodextrin.