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polypeptide containing a signal peptide which is cleaved off by an envelope protease.1) The amino acid composition2) and the sequence of N-terminal five residues3) of the signal peptide were determined. The synthesis of alkaline phosphatase is induced by phosphate star vation and is regulated by phoR (negatively) and phoB (positively) which are both located near the structural gene phoA at 8.5 min of the genetic map. 4) We are interested in this enzyme because (a) the mechanism of biosynthesis and excretion of periplasmic enzyme can be analysed, (b) the regulation of its expression remains yet to be elucidated, and (c) its promoter and signal sequence is promised to be capable of mobiliz ing proteins of interest through the cytoplas mic membrane into the periplasm on condition the structural genes are appropriately fused through techniques of gene manipulation. In this communication, we report the construc tion and the characterization of the plasmids carrying phoA gene. First, we constructed phoA-transducing 2

Cloning of the structural gene of alkaline phosphatase of Escherichia coli K12.