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Protoplasts of Brevibacterium flavum cells were obtained by treatment with 0.3 units of penicillin per ml during exponential growth, and followed by lysozyme treatment in a hypertonic medium. Protoplasts of antibiotic-resistant and auxotrophic strains were fused by a modified polyethyleneglycol technique, and allowed to revert to bacillary form on selective media. The clones were analyzed and recombinants were found not to segregate.

Fusion of protoplasts and genetic recombination of Brevibacterium flavum.