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The cell-free extract of Aspergillus niger (AKU 3302) catalyzed the degradation of nicotinamide mononucleotide to an unidentified compound. This compound was isolated from the reaction mixture and identified as nicotinic acid ribonucleoside (NAR). When the mold was cultured on media containing nicotinic acid- and nicotinamide-14C, the ratio of radioactivity incorporated into NAR was 16 and 10% of the total activity in the mycelial extracts, respectively.

Formation of nicotinic acid ribonucleoside by an enzyme preparation and growing mycelium of Aspergillus niger.