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Unsaturated long-chain fatty acids such as oleic, linoleic and ricinoleic acid except for elaidic acid were effective on the lipase production by Candida paralipolytica, but saturated fatty acids were not effective. When elaidic and myristic acids were dissolved in n-hexadecane to be dispersed in a liquid state, they became to be effective on the lipase production. These results suggest that long-chain fatty acids of a liquid state are effective on the lipase production. Lipase activator A and phosphatidylethanolamine stimulated the effect of fatty acids on the lipase production. Effects of sterols and surfaceactive substances on the lipase production were also reported. A weak tributyrin-hydrolyzing activity, in the absence of fatty acids, was found in the yeast cell.

Effects of fatty acids, lipase activator, phospholipids and related substances on the lipase production by Candida paralipolytica.