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In a previous paper," authors have demon strated that soybean protein bodies contained RNAs which consisted of tRNA and H-RNA. H-RNA contained 9S and 16S RNA com ponents and showed template activity in E. coli S-30 system. However, there still re mained a problem whether the template activity of H-RNA preparation is due to rRNA or mRNA in the preparation. In the present study, the template activities of crude rRNA or denatured rRNA from soybean ribosomes were examined and compared with that of H-RNA from protein bodies. And also purification of ribosomes by washing with EDTA or ammonium chloride solution was attempted to eliminate mRNA species from rRNA preparation, and the template activity of purified rRNA was examined. From the results, the template activity of H-RNA from protein bodies is discussed.

Some Aspects of the Template Activity of High Molecular Weight RNA from Soybean Protein Bodies