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During the course of the induction of many species of plant callus, it was found that callus of poplar, induced on the agar medium containing 0.5 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D), upon exposure to white light produces an anthocyanin in its cell. The callus was inoculated into submerged cultures under light on a reciprocal shaker, and the pigment produced was isolated as pure crystals.By the use of IR spectrum, UV spectrum and other physical and chemical methods, the anthocyanin was identified as cyanidin-3-monoglucoside (chrysanthemin).

Isolation and Identification of an Anthocyanin from the Cell Suspension Culture of Poplar