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The influences of moisture content of barley (Hordeum vulgare L.) seeds which were presoaked for 13 h and re-dried before irradiation on boron addition effect (BAE) and relative biological effectiveness (RBE) of alpha particles and protons were evaluated. Seeds of normal (10.21% in embryo), low (2.55%) and high (28.0%) moisture content showed different regression of BAE values on the absorbed amount of 10B. The regression coefficient was highest for normal moisture content, followed by low moisture content, and the lowest for high moisture content. RBE of alpha particles also significantly differed between the moisture contents. Seeds of normal, low and high moisture contents showed 46.4, 37.4 and 17.0 of RBE value, respectively. Contrarily, RBE values of protons did not significantly vary with moisture content. It was found that the ratio of RBE of alpha particles between different moisture contents could be expressed by the product of the three ratios, i.e. ratio of sensitivity to gamma-rays, ratio of BAE, and ratio of moisture content. It was concluded that adjustment of moisture of the seeds to normal content (about 10%) is important to get a high value of both BAE and RBE of alpha particles.

Moisture content of seeds affects relative biological effectiveness of alpha particles but not protons in thermal neutron exposure