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Sweetpotato (Ipomoea batatas (L.) Lam.) is a major crop that ranks seventh among food crops in annual production in the world (FAOSTAT database, May 2001). It would be important to further improve the quality of sweetpotato by applying genetic engineering technology, in addition to the use of traditional breeding methods. Recently, transgenic sweetpotato plants with genes introduced for insect and virus disease tolerance have been reported (Newell et al. 1995, Murata et al. 1997, Morán et al. 1998). We developed an efficient method for the production of transgenic sweetpotato plants through Agrobacterium tumefaciens-mediated transformation using embryogenic calli (Otani et al. 1998) and produced transgenic sweetpotato plants with genes for key enzymes of the metabolic pathway such as the granulebound starch synthase gene (GBSSI) to modify the amylose and amylopectin contents of starch (Kimura et al. 2001), and the ω-3 fatty acid desaturase gene isolated from tobacco (NtFAD3) to modify the fatty acid composition of sweetpotato plants (Wakita et al. 2001). Recently, transgenic crop plants expressing herbicide tolerance have been commercialized, because the use of non-selective herbicides such as glufosinate and glyphosate provides economically superior weed control. The bar gene encoding the enzyme, phosphinothricin acetyltransferase (PAT), that confers resistance to the commercial herbicides, glufosinate and bialaphos, was isolated from the bialaphos biosynthetic pathway of Streptomyces hygroscopicus (Murakami et al. 1986). Glufosinate-resistant transgenic crops, for example, Brassica napus (DeBlock et al. 1989), corn (Gordon-Kamm et al. 1990), and rice (Cao et al. 1992), have been produced. Weed control in sweetpotato fields after planting of cut sprouts is critical for high productivity. The application of genetic manipulation techniques to sweetpotato should enable the development of herbicide-resistance that otherwise would be difficult to achieve by the implementation of conventional breeding programs. Transgenic herbicide-resistant sweetpotato may enable to control weeds in cultivation more conveniently and more economically. We have introduced the bar gene into embryogenic calli via Agrobacterium tumefaciens and produced transgenic sweetpotato plants. In this paper, we describe the expression of the bar gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter or the modified CaMV35S promoter, El2Ω promoter (Mitsuhara et al. 1996) and subsequent tolerance of the transgenic sweetpotato plants to the herbicides.

Production of Herbicide-Resistant Seetpotato(Ipomoea batatas(L.)Lam.) Plants by Agrobacterium tumefaciens-mediated Transformation