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The crude extracts from leaves of Jatropha glauca plant grown naturally at Al-Baha region obtained by extraction with ethanol and fractionated using three solvents: petroleum ether, ethyl acetate and methanol were investigated for their in vitro antimicrobial susceptibility. The antimicrobial activity were evaluated against two bacterial strains S. aureus and E. coli, and two common fungal strains Candida albicans and Candida krusei using disc diffusion assay. The crude ethanolic extract and its fractions methanolic and ethyl acetate showed an inhibitory effect against both bacterial and fungal microorganisms. The petroleum ether fraction had no antimicrobial effect. The ethanolic crude extract and methanolic fraction showed higher zone of inhibition, while the ethyl acetate fraction showed the least activity against both bacterial strains. The methanolic fraction was found to possess lowest MIC ≥250 μgml−1 against both tested bacterial strains. Similarly, the ethanolic crude extract and methanol fraction showed zone of inhibition against tested fungal strains with methanolic fraction showed the highest zone of inhibition. However, these observations indicated that the ethanolic crude extract and its methanolic and ethyl acetate fractions exhibited some antimicrobial potency that proves the leaves contain some gradients that have antibacterial and antifungal potential.

world journal of organic chemistry

&lt;i&gt;In&lt;/i&gt;&lt;i&gt; &lt;/i&gt;&lt;i&gt;vitro&lt;/i&gt; Antimicrobial Efficiency of Crude Extracts of Wild&lt;i&gt; &lt;/i&gt;&lt;i&gt;Jatropha&lt;/i&gt;&lt;i&gt; &lt;/i&gt;&lt;i&gt;glauca&lt;/i&gt; Plant Leaves Grown Naturally at Al-Baha Region, KSA