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This study was carried out to determined the variation in microbiota of five section of Igbinedion University Teaching Hospital Okada. Samples were collected using the settled plate techniques for the enumeration of bacterial and fungal isolates. The air specimens were collected three times; in the morning between the hours of 8 am and 10 am, in the afternoon between 12 noon and 2 pm and in the evening between the hours of 4pm and 6pm. The total heterotrophic microbial population of the five different units (wards) studied varied from wards to wards. The highest bacterial population was recorded in the afternoon between time 12 pm and 2 pm compared to the morning and evening, ranging from 5 cfu/m3 to 17 cfu/m3, with the accident and emergency ward recording the highest bacterial counts of 17 cfu/m3. The concentration of fungal population in air of the five different wards also followed the bacteria pattern in which highest fungi population were recorderd in afternoon (16 cfu/m3). At the three different times of study, the Accident and emergency ward, male ward, female ward as well as children wards show high yield fungal isolates. Five bacteria were isolated, namely; Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes and Proteus mirabilis and identified accordingly. Aspergillus sp, Penicillum sp, and Mucor sp, were three fungi genera isolated. The degree of frequency of microbial distribution was high in the accident and emergency ward but lower in operating room (theatre). Accident and emergency ward my serve as source of infection in this hospital hence there should be an increase in level of hygiene in this unit also human movement should be limited scince they often serve as souces of pathogen.

Indoor Airborne Microflora in Various Section of a Tertiary Healthcare Centre in Rural Area of Ovia Northeast Edo State