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We present a genome assembly from an individual female  Nymphalis polychloros  (the large tortoiseshell; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The genome sequence is 398 megabases in span. The majority of the assembly is scaffolded into 32 chromosomal pseudomolecules, with the W and Z sex chromosome assembled.

The genome sequence of the large tortoiseshell, Nymphalis polychloros (Linnaeus, 1758)