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Here we describe the development of a protocol to make small oligomers, dimers and trimers, from highly oligomeric membrane proteins. The proteins that we used are the light harvesting 2 proteins and core complexes from photosynthetic bacteria, which contain respectively 16 and 56 individual polypeptides. Creating specific dimers between such multimeric protein poses several problems. We propose a protocol based on asymmetric lysine localization, thanks to the positive inside rule, and copper-free click chemistry. With this method we are able to produce specific dimeric complexes in detergent solution of possible biological relevance.

Making dimers of oligomeric membrane proteins using copper-free click chemistry