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https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

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Background:   Uncaria  gambir is one  Uncaria  species that exclusively grows in Indonesia. The phytochemical constituents of this species have been widely explored and its extracts are used as traditional medicine. However, the relationship between  Uncaria gambir  and other  Uncaria  species is still unknown. DNA barcoding was used in this study to reveal this relationship.    Methods:  Genomic DNA was isolated from four main cultivated variants of  Uncaria gambir  species in Indonesia. ITS primer was used to amplify the specific gene region. Genetic distance analysis was carried out on  Uncaria gambir  and 12 other  Uncaria  species. A phylogenetic tree was created to determine the relationship among  Uncaria  species using the maximum likelihood method.    Results:  The ITS primer successfully amplified the ITS region in  Uncaria gambir . Genetic distance and phylogenetic tree analyses showed that  Uncaria gambir  has a close relationship with  Uncaria scandens ,  Uncaria yunnanensis , and  Uncaria macrophylla  which is also indicated by Interspecific distance analysis.    Conclusions:  Although the DNA barcoding gap is absent in genetic distance analysis, the phylogenetic tree analysis from the ITS region can differentiate  Uncaria gambir  from other  Uncaria  species.

DNA barcoding for the discrimination of Uncaria gambir and its closely related species using internal transcribed spacer genes