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Background:  Bacteria can adhere and grow on any surface due to their chemical and physical interaction, leading to the development of biofilms. Essential oils have a great potential for use in the food industry, as they can effectively prevent the presence of some pathogenic microorganisms.    Species such as those in the  Bacillus  genus have the ability to produce toxins. Some strains of  Bacillus subtilis  have been related to cases of food‐borne diseases. In the bakery industry,  B. subtilis  also has been related to “rope” disease, linked to bread preservation processes.    Methods:  The aim of the study was to analyse the antibacterial properties of 24 chemotyped essential oils against the growth of  B. subtilis . The biological activity study was carried out using disk diffusion in agar and broth dilution methods.    Results:  The essential oil of  Cymbopogon martinii var. motia  had a high geraniol content (&gt;80.53%) and showed a high antimicrobial effect against the Gram-positive bacterium  B. subtilis . Binary combinations of  Cymbopogon martinii var. motia  oil with  Eugenia caryophyllus  showed antagonistic effects on  B. subtilis .    Conclusions:   The essential oil of  Cymbopogon martinii var. motia  has an interesting potential use in the bakery industry as a preservative, in applications such as nano encapsulation for bakery doughs, active packaging of baked products, or surface disinfectants.

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Essential oil of Cymbopogon martini, source of geraniol, as a potential antibacterial agent against Bacillus subtilis, a pathogen of the bakery industry