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Terminal degeneration of the rat spinal cord after the transection was investigated using the ZIO staining method. Degenerating processes were classified into three types according to ZIO reactivity of mitochondria, synaptic vesicles, synaptic membrane and matrices of degenerating terminals. It was established that ZIO staining was useful not only for the detection of degenerating terminals but also for the determination of degenerating stages.

ZIO STAINING FOR DEGENERATING TERMINALS OF RAT SPINAL CORDS AFTER TRANSECTION