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Growth cartilage (GC) cells comprise distinct cellular zones of proliferating, hypertrophic and calcifying stages. To obtain representative cell clones of each GC stage, the author immortalized primary cultured rat GC cells by retro virus carrying neo gene, cytomegalo virus promoter and c-myc oncogene. The GC cells were freed from rat costal GC by enzymatic digestion. Retro virus carrying neo gene, cytomegalo virus promoter and c-myc oncogene was infected onto the cultured GC cells. After screening and cloning, 22 clones with different characteristics were obtained, and examined for their morphology, nodule formation, proteoglycan formation, alkaline phosphatase activity, fiber formation, reactivity to late GC specific antibody and response to 1-34 PTH. Depending on these examined characteristics, they were assessed as fibroblasts (2 clones), resting cartilage cells (3 clones), or as GC cells (5 clones as proliferating GC cells, 5 clones as early hypertrophic GC cells, 6 clones as late hypertrophic GC cells, and 1 clone thought to be either late hypertrophic or calcifying GC cell).

Immortalization of Rat Growth Cartilage Cells by Retro Virus Carrying C-myc Oncogene and Their Histochemical and Immuno-Histochemical Characteristics.