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(ACFEM) developed recently, it is possible to localize cholesteryl ester in lipid droplets (LD) directly. Using the ACFEM, cathodoluminescence (CL) from the adrenocortical cells have been investigated (2, 3, 5). Nakano et al. (5) have reported that 14 days after hypophysectomy the CL of 320 nm wavelength was significantly increased and that of 430 nm wavelength diminished. In this study we have examined by the ACFEM the time course of changes of the CL from adrenocortical cells of hypophysectomized animals from 2 days to 23 days after operation especially focusing on the diminishing of the CL of 430 nm wavelength. Male Wistar rats from Imamichi Institute of Animal Reproduction (Saitama, Japan) and Japan SLC Inc., were divided into the following groups: 1. Untreated control rats, 2. Hypophysectomised rats, decapitated at 2, 5, 7, 14 and 23 days after operation. The completeness of hypophysectomy was determined at sacrifice by inspection of sella turcica under a dissecting microscope. Both adrenal glands were removed, rinsed in ice-cold phosphate buffered saline and fat was trimmed from them. Adrenal cortex was sectioned into small pieces, and then put on small metal cryotips, rapidly frozen in precooled liquid propane and cut by a cryo-ultramicrotome (Ultracut E, FCi4; Reichert) to make the surface of the specimen flat for observation in the ACFEM. After obtaining the color CL images and the CL spectra, thin coating with gold was performed inside the specimen chamber of the electron microscope to observe secondary electron images. In adrenocortical cells of control rats the CL ap-

Effect of hypophysectomy on rat adrenocortical cells studied by analytical color fluorescence electron microscopy.