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The protein kinase A was demonstrated at the electron microscopic level in the rat hippocampus by an immunocytochemical method. The enzyme was localized in neurons as well as in filial cells. Immunocytochemical reactions were found in the cytoplasm of the cells, on the endoplasmic reticulum and in nuclei. Strong staining was detected in synapses, in the postsynaptic thickenings and on the microtubules of dendrites. No staining was found on axonal microtubules and in oligodendrocytes. Astroglial cells were regularly stained, somata as well as processes contained reaction product.

Immunohistochemical demonstration of protein kinase A in the rat hippocampus.