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Mast cells are known to be a type of free cell in the connective tissues of vertebrates, and to contain a number of basophilic granules in their cytoplasm (7, 19) . Although the chemical compositions of these granules vary with animal species, they are reported to contain heparin, histamine, serotonin, hyaluronic acid, various peptides and enzymes such as platelet activating factor, slow reacting substance of anaphylaxis, different kinds of proteases, decarboxylases and certain hydrolases (5, 7, 13, 19). Of these components, heparin is known to be a sulfated glycosaminoglycan which exhibits anticoagulating activity in its physiological function. Recent biochemical studies on the cytoplasmic granules of mouse mast cells in mouse mast cell-fibroblast monolayers (1) and mouse bone marrow-derived mast cells (16-18) revealed that sulfated glycosaminoglycans contained within the granules were chondroitin sulfates, in addition to heparin. More recently, a biochemical analysis of rat mucosal mast cells clarified that the major polysaccharide produced by the cells is an oversulfated galactosaminoglycan rather than heparin (4). It remains to be determined, however, whether such dual composition of sulfated glycosaminoglycans of mast cell granules can be substantiated by cytochemical means. In the present study, attempts have been made to cytochemically analyze sulfated glycosaminoglycans in the cytoplasmic granules of mast cells from bovines and rats. In keeping with the results of previous biochemical studies (1, 4, 16-18), the results obtained in the present study have substantiated the dual composition of sulfated glycosaminoglycans of the mast cell granules, and this appears to be provocative of further cytophysiological studies on the precise functions of this particular cell type in the connective tissues.

Cytochemical analysis of sulfated glycosaminoglycans in mast cell granules of certain mammalian species.