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The histological staining method for HBs Ag is useful for the diagnosis of non-A, non-B hepatitis because the diagnosis of non-A, non-B hepatitis can be made only by ruling out the presence of other viral agents, including hepatitis B virus, hepatitis A virus, cytomegalovirus, herpes simplex virus, and Epstein-Barr virus. Many methods have been developed for the purpose of staining HBs Ag in paraffin sections, including an orcein method (11), modified orcein method (8, 9), aldehyde fuchsin method (11), aldehyde thionine method (11), Victoria blue method (16), and resorcin fuchsin method (8). Among these Shikata's orcein method was the first one that used dyes, and has been widely employed. This method was compared with the immunofluorescent method (11, 16) and the material stained with orcein and Victoria blue was suggested to be HBs Ag. The purpose of this paper is to present HBs Ag staining dye reactions in vitro, interpretation of nonspecific reactions which appeared in orcein staining, comparison of five HBs Ag staining methods, and notes of the HBs Ag procedure.

Histologic staining methods for hepatitis B surface antigen (HBS AG) and interpretation of dye reactions in vitro.