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Compatibility mutants such as Hfr-i, Hfr-i' and Hfr- were isolated from Hfr (Hayes' strain) by acriflavine treatment. Hfr-i, Hfy-i' and Hfr- resemble F+, Fi and F- respectively in their mating behavior, possession or lack of transmissible agent and susceptibility to infection by F. There is no difference, between Hfr-i and F+ in their mating behavior, the role as chromosome donor in recombination and possession of transmissible agent, and there is also no difference between Hfr- and F- in their mating behavior, the role as chromosome recipient in recombination and susceptibility to infection by F within the limits of the technique empolyed.

Artificial F elimination in Escherichia coli K-12: The effects of acriflavine on Hfr strain