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Shells of Bithynia: widely distributed B. tentaculata (Linnaeus), Balkan B. cettinensis Clessin from Cetina River in Croatia, and B. zeta Glöer et Pešić from Vitoja spring at Lake Skadar, Montenegro, as well as male genitalia of B. zeta and B. cettinensis were examined. The shells of all three taxa are similar, also the penes differ only slightly. The mean value of the length ratio of the tubular penial gland (measured along its curvature) to the penis right arm, measured along the curvature of its right margin, was 1.63 for B. zeta, and 1.33 for B. cettinensis. Those values differed slightly, especially compared to 5.0–5.9 for B. tentaculata. In the maximum likelihood (ML), as well as Bayesian (BI) trees, B. cettinensis and B. tentaculata were sister clades with p-distance of 0.007, and B. zeta was more distinct, with p-distance of 0.122 to B. cettinensis and 0.154 to B. tentaculata. The species distinctness of the three studied taxa was confirmed. key wordS: species distinctness, shell, penis, tubular penial gland, mtDNA, COI

Species distinctness of Bithynia cettinensis Clessin, 1887 and B. zeta Glöer et Pešić, 2007 (Caenogastropoda: Truncatelloidea)