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Shell habitus and COI mitochondrial gene sequences of one freshwater snail from Khios and three from Rhodes islands were analysed. Both methods confirmed assignment of these specimens to the genus Daphniola Radoman, 1973. Genetic distance between individuals from these two islands is surprisingly low, strongly suggesting that they belong to the same species, still undescribed. Comparison of COI sequences with other known species of this genus shows that the closest relative of the Khios and Rhodes populations is D. louisi Falniowski et Szarowska, 2000 from Attica. The results are discussed in the context of geological and climatic history of the Mediterranean.

Daphniola Radoman, 1973 (Caenogastropoda: Truncatelloidea) at east Aegean islands