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Clone libraries were used to evaluate the effects of 2,4-dinitroanisole (DNAN) and n-methyl-4-nitroaniline (MNA) on bacterial populations within three anaerobic bioreactors. Prior to the addition of DNAN and MNA greater than 69% of the clones in each reactor were identified as a single Desulfuromonales species. However, after 60 days of treatment the Desulfuromonales distribution decreased to less than 13% of the distribution and a clone identified as a Levilinea sp. became the dominant organism at greater than 27% of the clone distribution in each reactor suggesting the species may play an important roll in the reduction of DNAN and MNA.

microbes and environments

Analysis of Bacterial Community Diversity in Anaerobic Fluidized Bed Bioreactors Treating 2,4-Dinitroanisole (DNAN) and n-Methyl-4-nitroaniline (MNA) Using 16S rRNA Gene Clone Libraries