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Miyagi University of Education, Aramaki-aza-Aoba, Aoba-ku, Sendai 980–0845, Japan(Received October 7, 2006—Accepted November 29, 2006)A PCR primer set was designed to amplify the 18S rRNA gene from the phylum Ciliophora (ciliates). Thepair amplified DNA from 5 organisms representing the major taxonomic groups of ciliates but not from 13 non-ciliate organisms. To test the ability of the primers to amplify ciliate rRNA genes from environmental samples, aclone library was constructed from an agricultural soil sample and analyzed. The rRNA gene sequences of allclones were affiliated with the Ciliophora. This newly developed PCR primer set is able to amplify ciliate rRNAgenes from soil, and may be useful for the detection and identification of ciliates.

A Taxon-specific Oligonucleotide Primer Set for PCR-based Detection of Soil Ciliate