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Tropospheric increment of N2O is of great concern in global warming issue and stratospheric ozone chemistry, which is largely attributed to the human-induced alteration of nitrogen cycle. Biological waste water treatment is a terminal destination of industrially fixed nitrogen as well as a hot spot for N2O emission. The present understanding of microbial pathways suggests that N2O is a by-product of ammonium oxidation and an obligate intermediate product of denitrification. Therefore, a conventional mass balance approach is insufficient to elucidate producing mechanisms of N2O in common environments where coupling of nitrification and denitrification occurs. In this context, we proposed the measurement of natural abundance of nitrogen and oxygen isotopes to be applied for analysis of N2O dynamics in a waste water treatment facility, and discussed future perspective of the technique.

Elucidation of Biological N2O Emission Mechanisms and Its Significance in N2O Dynamics in a Waste Water Treatment Facility.