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Histochemical examinations of proteins, neutral and acid polysaccharides, and lipids were made using the oocytes in primordial, secondary and antral follicles and the embryos at the stages of 1to 8-cell, morula and blastocyst of the mastomys, Praomys natalensis. The cytoplasm and zona pellucida of both oocytes and embryos always contained acrolein-Schiff reactive proteins, but not sudanophilic lipids. The zona pellucida of both oocytes and embryos contained neutral polysaccharides that were periodic acid-Schiff reactive and salivary test-resistant, and alcian blue positive acid polysaccharides, while the cytoplasm of those possessed no such substances. The cytoplasm of oocytes in secondary and antral follicles and of embryos at the stages of l-cell to blastocyst possessed a large amount of glycogen granules that were periodic acid-Schiff reactive and salivary test-digestible.

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Histochemical Observations of Proteins, Polysaccharides and Lipids in the Oocytes and Embryos of Mastomys(Praomys natalensis)during the Folliculogenesis and Early Development.