English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Endotoxins released by Gram-negative bacteria are potent stimulators of tumor necrosis factor α (TNF-α) production. The objectives of this study were to evaluate plasma levels of TNF-α, TNF-α secretion, and mRNA levels of TNF and TNF-α receptor type 1 (TNFR1) following exposure to lipopolysaccharide (LPS). For this, we used cultured endometrial cells or organ cultures, throughout the estrous cycle, after hormone treatment with medroxyprogesterone acetate (MPA), and during pyometra. Plasma TNF-α concentrations were increased in animals at estrus (P &lt; 0.05) compared to other groups. In the LPS-challenged endometrium, secretion of TNF-α by tissues collected during estrus increased (P &lt; 0.001) compared to that of other groups. LPS, alone or combined with TNF-α, upregulated TNF gene expression in the feline endometrium at diestrus (P &lt; 0.001 for both treatments), in queens treated short-term with MPA (P &lt; 0.01 and P &lt; 0.05, respectively) and in queens treated long-term with MPA (P &lt; 0.01 and P &lt; 0.001, respectively). During pyometra, TNF and TNFR1 mRNA were increased only after tissues were challenged with TNF-α and LPS (P &lt; 0.001 and P &lt; 0.01, respectively). When cultured endometrial cells were challenged with LPS, the concentration of TNF-α increased only in epithelial cells after 4 h and 12 h (P &lt; 0.05 and P &lt; 0.01, respectively). Since LPS did not affect stromal cells, but TNF-α increased its own transcript after 2 h (P &lt; 0.01), 4 h (P &lt; 0.05) and 12 h (P &lt; 0.001), we assume that stromal cells are not directly involved in pathogen recognition, as was the case for epithelial cells.

journal of reproduction and development

Comparison of the effect of lipopolysaccharide on tumor necrosis factor α (TNF-α) secretion and &lt;i&gt;TNF&lt;/i&gt; and &lt;i&gt;TNFR1&lt;/i&gt; mRNA levels in feline endometrium throughout the estrous cycle during pyometra and after medroxyprogesterone acetate treatment