Open AccessAn siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei
Author(s) -
Appolinaire Djikeng,
Huafang Shi,
Christian Tschudi,
Shuiyuan Shen,
Elisabetta Ullu
Publication year - 2003
Publication title -
rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.037
H-Index - 171
eISSN - 1469-9001
pISSN - 1355-8382
DOI - 10.1261/rna.5270203
Subject(s) - biology , small interfering rna , polysome , rna interference , rna silencing , trans acting sirna , trypanosoma brucei , ribonucleoprotein , rna , ribosome , microbiology and biotechnology , dna directed rna interference , genetics , gene
Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei , 10%–20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.
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