Open AccessSequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
Author(s) -
Gunter Meister,
Markus Landthaler,
Yair Dorsett,
Thomas Tuschl
Publication year - 2004
Publication title -
rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.037
H-Index - 171
eISSN - 1469-9001
pISSN - 1355-8382
DOI - 10.1261/rna.5235104
Subject(s) - biology , microrna , rna interference , gene silencing , rna , rna silencing , trans acting sirna , non coding rna , computational biology , small interfering rna , function (biology) , microbiology and biotechnology , genetics , gene
A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.
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