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The biogenesis of transfer RNA is a process that requires many different factors. In this study, we describe a genetic screen aimed to identify gene products participating in this process. By screening for mutations lethal in combination with a  sup61-T47:2C  allele, coding for a mutant form of   \batchmode\documentclass[fleqn,10pt,legalpaper]{article}\usepackage{amssymb}\usepackage{amsfonts}\usepackage{amsmath}\pagestyle{empty}\begin{document}\(\mathrm{tRNA^{Ser}_{CGA}}\)\end{document}  , the nonessential  TAN1  gene was identified. We show that the  TAN1  gene product is required for formation of the modified nucleoside  N  4 -acetylcytidine (ac 4 C) in tRNA. In  Saccharomyces cerevisiae , ac 4 C is present at position 12 in tRNAs specific for leucine and serine as well as in 18S ribosomal RNA. Analysis of RNA isolated from a  tan1 -null mutant revealed that ac 4 C was absent in tRNA, but not rRNA. Although no tRNA acetyltransferase activity by a GST-Tan1 fusion protein was detected, a gel-shift assay revealed that Tan1p binds tRNA, suggesting a direct role in synthesis of ac 4 C 12 . The absence of the  TAN1  gene in the  sup61-T47:2C  mutant caused a decreased level of mature   \batchmode\documentclass[fleqn,10pt,legalpaper]{article}\usepackage{amssymb}\usepackage{amsfonts}\usepackage{amsmath}\pagestyle{empty}\begin{document}\(\mathrm{tRNA^{Ser}_{CGA}}\)\end{document}  , indicating that ac 4 C 12  and/or Tan1p is important for tRNA stability.

The Saccharomyces cerevisiae TAN1 gene is required for N4-acetylcytidine formation in tRNA

The Saccharomyces cerevisiae TAN1 gene is required for N⁴-acetylcytidine formation in tRNA