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Effects of Astragalus polysaccharide (APS) and Radix hedysari polysaccharide (RHPS) on the third component of complement (C3) cleavage production of macrophages in ICR mouse were investigated by the immunofluorescent method. By two hours after intraperitoneal injection of 500 mg/kg of APS or RHPS, there was an obvious increase in the deposition of C3 on peritoneal macrophages. When APS or RHPS was injected 5 times (1 time/day), the proportion of C3 positive macrophages was more than that of 1-time injection. At the same time, the number of peritoneal macrophages also increased. Since C3 cleavage product occurred with the activation of C3, the results suggest that the immuno-potentiating action of APS and RHPS may be related to the activity of mouse complement C3.

Enhancing effect of antitumor polysaccharide from Astragalus or Radix hedysarum on C3 cleavage production of macrophages in mice.