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The experiment to be reported is an attempt to investigate the mechanism of action of cortisol in the Henle's loop. Male albino rats weighing 170 to 270 g were adrenalectomized and given free access to 0.9% saline. Six to seven days after adrenalectomy, five milligram of cortisol (alchohol free) was injected subcutaenously for 2 days previously. Additional cortisol (5 mg) was injected subcutaneously 60 minutes prior to the start of the experiment. The method of this experiment was almost same as that previously reported (1). A proximal tubule of anesthetized rat (Nembutal 30 mg/kg i.v.) was punctured with a perfusion pipette and a sample was collected from the first part of the distal segment of the surface of the kidney. Ringer's solution (NaCl; 147 mM KCl 4.02 mM, CaC12i 2.7 mM) containing inulin (0.15-0.20%) and lissamine green 0.05%) was used as perfusate and perfusion rate was 19 nl/min. The osmolality of this solution was 302 mOsm/L. The osmolality, Na and inulin concentration of the samples were measured with a micro-osmometer, an ultramicroflamephoto meter (Erma Model 677) and micro-fluorophotometer (AMINCO) (2). These values were shown as a TF/p

EFFECTS OF CORTISOL ON THE HENLE’S LOOP OF THE ADRENALECTOMIZED RAT’S KIDNEY