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The facts that morphine inhibits the release of ACh from isolated guinea-pig intestine and also it suppresses the ChE activity, in vitro or in vivo, led to the hypothesis that the cholinergic mechanism may be involved in the effect of morphine on the central nervous system.

EFFECT OF MORPHINE ON THE LIBERATION OF ACETYLCHOLINE FROM THE MOUSE CEREBRAL CORTICAL SLICES IN RELATION TO THE CALCIUM CONCENTRATION IN THE MEDIUM