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cium was omitted from the medium, and markedly inhibited by the addition of cocaine. 3. Addition of reserpine or tyramine stimulated the release of catecholamine from the slices. The effect of reserpine was diminished in calcium free medium, but that of tyramine was not affected in calcium free medium. Cocaine had a weak inhibitory effect on the release of catecholamine caused by reserpine but no effect on the response to tyramine. 4. Calcium ion itself stimulated the released of catecholamine from the granules, but its effect was prevented by magnesium. From the above results it is suggested that calcium ion plays a cardinal role in the

PHARMACOLOGICAL EVIDENCE FOR THE EXISTENCE OF SYMPATHETIC GANGLIA IN THE VICINITY OF THE VAS DEFERENS OF GUINEA PIGS