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The experimental ear burn model in mice was developed in order to research the wound surface on a burn injury biochemically, and the following results were obtained: 1) The ear weight tended to increase up to 3 hr after preparation of the burn and then decreased subsequently. 2) Significant increase in leakage of Evans blue (EB) dye into the ear was observed in the burn group as compared to the non-burnt group. The amount of EB dye leaked per ear became the maximum in 3 hr which was similar to the increase in ear weight. 3) Correlation between the ear weight after burn and the amount of EB dye leaked into the ear on the burned site was observed up to 6 hr after preparation of the burn. 4) Vasopermeability of the ear was depressed with antihistamine treatment, but non-steroidal anti-inflammatory drugs had no effect. The ear weight of glucocorticoid-treated mice tended to be decreased as compared with the weight of the burned ear. 5) The histamine contents in the burned ear did not change, but the bradykinin contents significantly increased after the burn.

Study on ear burn model in mice and its significant application.