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The short-circuit current (Ussing chamber) technique is one of the most powerful methods to measure transepithelial ion transport across a variety of epithelial membranes including intestinal mucosa. This method is very useful for many researchers in physiology and pharmacology. This review article shows the theory and practice of the short-circuit current technique.

Electrophysiological measurement of transepithelial ion transport: short-circuit current (Ussing chamber) technique.