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Titers of antideoxyribonuclease-B (ADNB, by Streptonase-B test), antistreptolysin-O (ASO, by Rantz-Randall's micromethod) and Kinase test (antivaridase agglutination test) were performed on 118 sera of patients with suspected streptococcal infection and compared. Between ASO and ADNB, a fairly significant correlation was seen (r = 0.69), but many cases in which ADNB exceeded ASO were observed. Between Kinase and ADNB, no significant correlation was seen (r = 0.49). Successive determination of antibody titers were made in 14 consecutive patients with acute glomerulonephritis, anaphylactoid purpura nephritis, rheumatic fever and chronic rheumatic heart disease. In general, ADNB titer was significantly higher than ASO with few exceptions. The duration of high titer of ADNB was much longer than ASO Kinase titers were sometimes unstable. Throughout this study it has been noted that the result of ADNB was very easily to read and was highly reproducible. There were no nonspecific or pseudopositive reactions. Therefore, ADNB is useful for the diagnosis of streptococcal infection and its complication especially when other antibody titers give negative or doubtful results.

Clinical appraisal of the antideoxyribonuclease-B (ADNB) by means of streptonase-B test (Wampole).