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In order to clarify the mechanism of topoisomerase II mediated DNA cleavage activity caused by intercalators (9-anilinoacridines), antitumor activities against murine P388 and calf thymus DNA topoisomerase II mediated DNA cleavage activities of compounds m-AMSA, A4P73, A3P56, and A3P166 have been studied. A4P73 was found to have potent antitumor and DNA cleavage activities.

Topoisomerase II Mediated DNA Strand Cleavage and Antitumor Activities against Murine Leukemia P388 of Novel Acridines